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Home⇒ Medical Science⇒ Biochemistry⇒ Uv luminance spectroscopy
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Internal conversion is where A molecule converts excess electronic energy to vibrational energy [other wrong options] [Discuss in forum] ![]() ![]() the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa) A molecule converts its excess energy to light, and emits a photon none of the above |
Q2 ⇒ Why do fluorescence spectrometers often use double-beam optics? To compensate for power fluctuations in the radiation source [other wrong options] [Discuss in forum] ![]() ![]() So a reference solution can be used To compensate for beam attenuation by the monochromator All of the above |
Q3 ⇒ What is the relationship between wavelength and wave number? Wavenumber = 1 / wavelength in centimeters [other wrong options] [Discuss in forum] ![]() ![]() Wavenumber - wavelength in nanometers = 1 Wavelength in nanometers x wavenumber = 1 None of the above |
Q4 ⇒ For a molecule to absorb IR, why must the molecule's vibrations cause fluctuations in the dipole moment of the molecule? Because for absorption to occur, the radiation must interact with the electric field caused by changing dipole moment [other wrong options] [Discuss in forum] ![]() ![]() Because a change in dipole moment lowers the energy required for electronic transitions Because fluctuations in the dipole moment allow the molecule to deform by bending and stretching All of the above |
Q5 ⇒ Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy? Because the magnitude of the output signal is proportional to the power of the incident radiation [other wrong options] [Discuss in forum] ![]() ![]() Because the sample won't fluoresce if the incident radiation is of low power To allow for scattering by the sample None of the above |
Q6 ⇒ Fluorescence occurs when a molecule returns to the electronic ground state from an excited singlet state by losing it's excess energy as a photon [other wrong options] [Discuss in forum] ![]() ![]() a molecule returns to the electronic ground state from an excited triplet state by losing it's excess energy as a photon a molecule lowers its vibrational energy by losing it's excess energy as a photon none of the above |
Q7 ⇒ Why phosphorescence measurements are usually made at a low temperature? To promote phosphorescence by slowing the rate of radiationless transfer processes [other wrong options] [Discuss in forum] ![]() ![]() To prevent thermal degradation of the phosphorescent species To increase the efficiency of the detector To decease the efficiency of detector |
Q8 ⇒ In the intersystem crossing the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa) [other wrong options] [Discuss in forum] ![]() ![]() a molecule converts its excess energy to light, and emits a photon a molecule converts excess electronic energy to vibrational energy all of the above |