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Home⇒ Medical Science⇒ Biochemistry⇒ Polymerase chain reaction
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Southern blotting involves electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation [other wrong options] [Discuss in forum] electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation electrophoresis of DNA molecules followed by incubation electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation |
Q2 ⇒ The viruses that can achieve neoplastic transformation are called DNA tumor viruses [other wrong options] [Discuss in forum] RNA tumor viruses retroviruses HIV none of these |
Q3 ⇒ To clone into a plasmid vector, both the plasmid and the foreign DNA are cut with the same restriction enzyme and mixed together [other wrong options] [Discuss in forum] with different restriction enzyme and mixed together with the combination of enzymes and then seperated with the combination of enzymes and mixed together |
Q4 ⇒ PCR is an abbreviation for Polymerase Chain Reaction [other wrong options] [Discuss in forum] Protein Change Reaction Positive Charge Reaction: Preliminary Change Rate |
Q5 ⇒ DNA fragments in a restriction digest can be separated by electrophoresis in both (a) and (b) [other wrong options] [Discuss in forum] poly acrylamide agarose gel none of these |
Q6 ⇒ Bacteriophages are bacterial viruses [other wrong options] [Discuss in forum] cells in the blood that eat bacteria a class of bacteria none of the above |
Q7 ⇒ For gene transfer to be effective, transforming DNA must be incorporated into the bacterial chromosome [other wrong options] [Discuss in forum] incorporated into a viral genome free in the bacterial cytoplasm none of the above |
Q8 ⇒ How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR? Four ds DNA molecule [other wrong options] [Discuss in forum] Two ds DNA molecule Three ds DNA molecule Eight ds DNA molecule |
Q9 ⇒ PCR can be used to amplify a specific fragment of DNA from which of the following? All of these [other wrong options] [Discuss in forum] A drop of blood A hair follicle A fragment of skin |
Q10 ⇒ The PCR, polymerase chain reaction is becoming the method of choice for screening gene [other wrong options] [Discuss in forum] Alteration of gene sterilization of gene all of these |
Q11 ⇒ PCR amplifies millions of copies of a short fragment of DNA [other wrong options] [Discuss in forum] the entire genome (all the DNA in a sample) both (a) and (b) short chain RNA |
Q12 ⇒ Which of the following is correct? All of the above [other wrong options] [Discuss in forum] HIV is a retrovirus that kills human helper T cells Causes acquired immune deficiency syndrome (AIDS) Cripples the immunity systems |
Q13 ⇒ In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to 16 single strands of DNA [other wrong options] [Discuss in forum] 16 double stranded DNA 18 single strands of DNA 18 double stranded DNA |
Q14 ⇒ PCR is used all of these [other wrong options] [Discuss in forum] to diagnose genetic diseases to solve crimes to study gene function |
Q15 ⇒ PCR (Polymerase Chain Reaction) can make multiple copies of DNA [other wrong options] [Discuss in forum] RNA protein carbohydrate |
Q16 ⇒ What technique can be used to determine the murderer who left blood with the victim? PCR amplification [other wrong options] [Discuss in forum] DNA sequencing Western blot RFLP mapping |
Q17 ⇒ From a single molecule of DNA, PCR can make millions of copies [other wrong options] [Discuss in forum] one additional copy hundreds of copies thousands of copies |
Q18 ⇒ Genomic libraries are made from genomic DNA of an organism [other wrong options] [Discuss in forum] genomic RNA of an organism genomic cDNA of an organism genomic mRNA of an organism |
Q19 ⇒ Restriction maps allows comparison between DNA molecules without the need to determine nucleotide sequence [other wrong options] [Discuss in forum] allows comparison between DNA molecules but requires to determine nucleotide sequence does not allow comparison between DNA molecules none of the above |
Q20 ⇒ Bactriophages absorb to a bacterial surface and inject the phage DNA through the cell wall into cytosol [other wrong options] [Discuss in forum] cell wall into plasmamembrane both (a) and (b) cell wall into endoplasmic reticulum |
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