Users Also Read
Read MCQ's/Objective questions
MCQ's Search Engine
Allosteric Effects
Amino Acid Metabolism
Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
Gel Electrophoresis
Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Lipid
Membrane Structure and Functions
Minerals
Nitrogen Metabolism
NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
Structure and Properties of Peptides
TCA Cycle
Thermodynamics and Free Energy
Transcription and Regulation
UV Luminance Spectroscopy
Vitamins and Coenzymes
Water, pH and Macromolecules
Home⇒ Medical Science⇒ Biochemistry⇒ Immunological techniques
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Immunoaffinity chromatography can be used in biochemical applications to purify protein antigen [other wrong options] [Discuss in forum] ![]() ![]() break down antibody structure break down antigen and analyze quantitatively none of the above |
Q2 ⇒ DNA- footprinting is a suitable technique for identifying which of the following? Protein binding site within DNA [other wrong options] [Discuss in forum] ![]() ![]() Particular mRNA in mixture Particular t-RNA in mixture Introns within DNA |
Q3 ⇒ Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid [other wrong options] [Discuss in forum] ![]() ![]() Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid |
Q4 ⇒ Which of the following is an immunodiffusion test? All of these [other wrong options] [Discuss in forum] ![]() ![]() Double-diffusion Gel diffusion Ouchterloney technique |
Q5 ⇒ What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) [other wrong options] [Discuss in forum] ![]() ![]() Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate |
Q6 ⇒ Which of the following radioactive element is generally involved in RIA? All of these [other wrong options] [Discuss in forum] ![]() ![]() Tritium Carbon-14 Iodine-125 |
Q7 ⇒ Which of the following is a positively charged matrix for ion exchange chromatography? DEAE cellulose [other wrong options] [Discuss in forum] ![]() ![]() CM cellulose Phosphocellulose None of these |
Q8 ⇒ The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Blue [other wrong options] [Discuss in forum] ![]() ![]() White Brown No colour is produced |
Q9 ⇒ Agarose a porous matrix used in various molecular biology technique is made by repetitive units of maltose [other wrong options] [Discuss in forum] ![]() ![]() agarobiose agar none of these |
Q10 ⇒ An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that there was more than one antigen [other wrong options] [Discuss in forum] ![]() ![]() the antibody was impure the temperature was too high there was one antibody and one antigen |
Q11 ⇒ What is the ELISA test intended to measure? Antibody to HIV only [other wrong options] [Discuss in forum] ![]() ![]() Antigen to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules |
Q12 ⇒ Rocket Immunodiffusion is also known as electroimmunodiffusion [other wrong options] [Discuss in forum] ![]() ![]() gel diffusion double-diffusion none of these |
Q13 ⇒ Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 20 kb and 2000 kb DNA [other wrong options] [Discuss in forum] ![]() ![]() 2000 kb and 20kb DNA 1000 kb and l0kb DNA 10 kb and 1000 kb DNA |
Q14 ⇒ Radial Immunodiffusion is similar to double-diffusion [other wrong options] [Discuss in forum] ![]() ![]() gel diffusion Ouchterloney technique all of these |
Q15 ⇒ What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate [other wrong options] [Discuss in forum] ![]() ![]() The ELISA would not develop when the substrate was added The ELISA would develop normally None of the above |
Q16 ⇒ In Counter immunoelectrophoresis electrophoresis will drive the antibody and antigen toward each other [other wrong options] [Discuss in forum] ![]() ![]() electrophoresis will drive the antibody and antigen parallel to each other the antibody will migrate towards anode the antibody will migrate towards cathode |
Q17 ⇒ What is approximatively the half life of 32-Phosphorus? 14 days [other wrong options] [Discuss in forum] ![]() ![]() 28 days 42 days 30 days |
Q18 ⇒ Which of the following is an immunoelectrophoresis technique? Both (a) and (b) [other wrong options] [Discuss in forum] ![]() ![]() Two-dimensional immunoelectrophoresis Counterimmunoelectrophoresis Coimmunoelectrophoresis |
Q19 ⇒ The competitive immunoassay can be used both (a) and (b) [other wrong options] [Discuss in forum] ![]() ![]() to detect very small amounts of antigen to detect antibody associated with allergies (IgE) commonly to detect trace amounts of drugs. |
Q20 ⇒ What radioactive molecule is used to end-label an oligonucleotide? (gamma)-32P-ATP [other wrong options] [Discuss in forum] ![]() ![]() (alpha)-32P-ATP (alpha)-32P-dUTP Any of these |
Or |