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Home⇒ Medical Science⇒ Biochemistry⇒ Ft ir spectroscopy
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ In FT-NMR, how are nuclei excited? By radio-frequency radiation whose frequency is swept across a predetermined range [other wrong options] [Discuss in forum] By an intense pulse of radiation which contains a wide range of frequencies By an intense pressure None of the above |
Q2 ⇒ An FT-IR instrument record a signal in the time domain [other wrong options] [Discuss in forum] frequency domain both (a) and (b) none of these |
Q3 ⇒ In scanning electron microscopy a specimen is fixed and then coated with thin layer of a heavy metal [other wrong options] [Discuss in forum] a specimen is fixed and then coated with transition metal a specimen is not fixed and then coated with thin layer of a heavy metal none of the above |
Q4 ⇒ Cytochrome C has an isoelectric pH of 10.05 [other wrong options] [Discuss in forum] 8.5 7.5 11.05 |
Q5 ⇒ Why is it advantageous to record many FID signals from the same sample and then add them together? To increase sensitivity [other wrong options] [Discuss in forum] To ensure that all target nuclei in the sample have been excited To remove inaccuracies caused by fluctuations in the applied magnetic field None of the above |
Q6 ⇒ In immunofluroscence microscopy, fluroscent compounds are attached to an antibody specific for the subcellular structure [other wrong options] [Discuss in forum] an antigen specific for the subcellular structure bound antibody specific for the subcellular structure none of these |
Q7 ⇒ What is meant by rotating frame of reference? If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space [other wrong options] [Discuss in forum] That the sample is spun rapidly in the applied field That the detector rotates around the sample None of the above |
Q8 ⇒ How do you turn a signal recorded in the time domain into a frequency domain signal? Fourier transformation [other wrong options] [Discuss in forum] Measurement of peak areas By use of a Michelson interferometer None of the above |
Q9 ⇒ What does the Michelson interferometer do? Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector [other wrong options] [Discuss in forum] Split a polychromatic beam of radiation into its component wavelengths Selectively filter certain wavelengths from a beam of I.R. radiation none of the above |
Q10 ⇒ The sequence of amino acids in proteins can be determined by means of all of the above [other wrong options] [Discuss in forum] identification of the -NH2 terminal amino acids identification of the -COOH terminal amino acids partial cleavage of the original polypeptide into smaller polypeptides |
Q11 ⇒ How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field? 2 [other wrong options] [Discuss in forum] 4 3 6 |
Q12 ⇒ What is the name given to the relaxation process due to an interaction between an excited nucleus and the magnetic fields caused by nuclei in molecules moving around in the sample? Spin - lattice relaxation [other wrong options] [Discuss in forum] Spin - spin relaxation Spin - spin - spin relaxation None of these |
Q13 ⇒ When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens? The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field [other wrong options] [Discuss in forum] The processional frequency of the nucleus increases The nucleus spins faster none of the above |
Q14 ⇒ Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis? Isoelectric point [other wrong options] [Discuss in forum] Molecular mass Solubility Folding |
Q15 ⇒ The frequency of precession, the transition frequency and the Larmor frequency are different terms for the same frequency [other wrong options] [Discuss in forum] same terms for the same frequency different terms for the different frequency same terms for the different frequency |
Q16 ⇒ Negative staining is a technique used in electron microscopy [other wrong options] [Discuss in forum] gel electrophoresis immunocytochemistry light microscopy |