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Home⇒ Medical Science⇒ Biochemistry⇒ Hplc
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ An isocratic elution in HPLC is one in which the composition of the solvent remains constant [other wrong options] [Discuss in forum] ![]() ![]() changes continuously changes in a series of steps none of these |
Q2 ⇒ Which can be used as a mobile phase in HPLC applications? Any compound with solubility in liquid [other wrong options] [Discuss in forum] ![]() ![]() Any compound with limited solubility in liquid Any compound with non-solubility in liquid Any of the above |
Q3 ⇒ Column efficiency is measured in terms of number of plates which is inversely related to the square of the peak width [other wrong options] [Discuss in forum] ![]() ![]() directly related to the square of the peak width inversely related to the cube root of the peak width directly related to the square of the peak width |
Q4 ⇒ In normal phase HPLC, there is a non polar solvent/polar column [other wrong options] [Discuss in forum] ![]() ![]() polar solvent/non-polar column non polar solvent/non-polar column any of the above |
Q5 ⇒ HPLC stands for both (a) and (b) [other wrong options] [Discuss in forum] ![]() ![]() High Pressure Liquid Chromatography High Performance Liquid Chromatography Highly Placed Liquid Chromatography |
Q6 ⇒ An eluotropic series ranks solvents by their relative abilities to displace solutes from a given absorbent [other wrong options] [Discuss in forum] ![]() ![]() ranks column packing material by their relative abilities to retain solutes on the column is a measure of the solvent adsorption energy none of the above |
Q7 ⇒ For a typical adsorbent such as silica gel, the most popular pore diameters are 60 and 100 A° [other wrong options] [Discuss in forum] ![]() ![]() 10 and 50 A° 100 and 150 A° 150 and 200 A° |
Q8 ⇒ Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)? All of the above [other wrong options] [Discuss in forum] ![]() ![]() Greater sensitivity and reusable columns Ideal for ionic species and large molecules Sample recovery |
Q9 ⇒ Which of the following statements is true for a refractive index detector in HPLC? It does not respond to many solutes [other wrong options] [Discuss in forum] ![]() ![]() It is more sensitive than a UV detector It can only be used for isocratic elutions none of above |
Q10 ⇒ HPLC methods include all of the above [other wrong options] [Discuss in forum] ![]() ![]() liquid/liquid (partition) chromatography liquid/solid (adsorption) chromatography ion exchange and size exclusion chromatography |
Q11 ⇒ Void volume refers to the total volume of eluent in the column the remainder being taken up by the packing material [other wrong options] [Discuss in forum] ![]() ![]() the volume of solvent contained in a liquid chromatographic column the time required for the gradient to reach the column the volume of the column between the point at which solvents are mixed and the beginning of the column |
Q12 ⇒ In reversed phase HPLC, there is a polar solvent/non-polar column [other wrong options] [Discuss in forum] ![]() ![]() non polar solvent/polar column non polar solvent/non-polar column any of the above |
Q13 ⇒ The eluent strength is a measure of solvent adsorption energy [other wrong options] [Discuss in forum] ![]() ![]() solvent absorption energy solvent diffusivity solvent mixing index |
Q14 ⇒ Dwell volume is defined as the volume of the column between the point at which solvents are mixed and the beginning of the column [other wrong options] [Discuss in forum] ![]() ![]() the volume of solvent contained in a liquid chromatographic column the time required for the gradient to reach the column none of these |
Q15 ⇒ A gradient elution in HPLC is one in which the composition of the solvent is changed continuously or in a series of steps [other wrong options] [Discuss in forum] ![]() ![]() remains constant both (a) and (b) none of the above |