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Home⇒ Medical Science⇒ Biotechnology⇒ Cloning vectors
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ A plasmid can be considered as a suitable cloning vector if All of the above [other wrong options] [Discuss in forum] it can be readily isolated from the cells it possesses a single restriction site for one or more restriction enzymes insertion of foreign DNA does not alter its replication properties |
Q2 ⇒ Charon 34 and Charon 35 can clone DNA upto 9-20 kb [other wrong options] [Discuss in forum] 1-2 kb 6-7 kb 30-35 kb |
Q3 ⇒ Size of the DNA that can be packaged into a λ phage is 35-53 kb [other wrong options] [Discuss in forum] 50 kb 40-50 kb any size |
Q4 ⇒ Cosmids lack genes coding for viral proteins [other wrong options] [Discuss in forum] origin of replication marker genes coding for replication cleavage site for the insertion of foreign DNA |
Q5 ⇒ Select the wrong statement about plasmids? Its replication depends upon host cell [other wrong options] [Discuss in forum] It is extrachromosomal It is double stranded It is closed and circular DNA |
Q6 ⇒ M 13 is an example of both (a) and (b) [other wrong options] [Discuss in forum] filamentous phage single stranded DNA vector plasmid |
Q7 ⇒ Cryptic plasmids do not exhibit any phenotypic trait [other wrong options] [Discuss in forum] exhibit many phenotypic traits exhibit one phenotypic traits exhibit antibiotic resistance |
Q8 ⇒ λ ZAP vector is an example of phagemid [other wrong options] [Discuss in forum] phage cosmid plasmid |
Q9 ⇒ P1 cloning vector is the example of bacteriophage [other wrong options] [Discuss in forum] plasmid cosmid phagemid |
Q10 ⇒ Cosmid vectors are used for cloning large fragments of DNA [other wrong options] [Discuss in forum] cloning small fragments of DNA cloning prokaryotic DNA only cloning eukaryotic DNA only |
Q11 ⇒ pBR 322 has/have which of the following selection marker(s)? Both (a) and (b) [other wrong options] [Discuss in forum] Ampr Tetr Kanr |
Q12 ⇒ P1 cloning vector allow cloning of DNA of the length of 100 kbp [other wrong options] [Discuss in forum] 50 kbp 20 kbp 10 kbp |
Q13 ⇒ EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 15-25 kb [other wrong options] [Discuss in forum] 6-7 kb 40-44 kb 1-2 kb |
Q14 ⇒ Cosmid vectors are plasmids that contain fragment of λ DNA including the cos site [other wrong options] [Discuss in forum] phages that lack cos site plasmids that have no selection marker cryptic plasmids |
Q15 ⇒ Maximum size of foreign DNA that can be inserted into a replacement vector is 20-25 kb [other wrong options] [Discuss in forum] 25-30 kb 18-20 kb 40-50 kb |
Q16 ⇒ Conjugative plasmids carry transfer genes called the tra genes [other wrong options] [Discuss in forum] exhibit antibiotic resistance do not exhibit antibiotic resistance do not carry transfer genes |
Q17 ⇒ Single stranded vectors are useful all of the above [other wrong options] [Discuss in forum] for sequencing of cloned DNA for oligonucleotide directed mutagenesis for probe preparation |
Q18 ⇒ Maximum size of foreign DNA that can be inserted into an insertion vector is 18 kb [other wrong options] [Discuss in forum] 35 kb 50 kb 27 kb |
Q19 ⇒ λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 6-7 kb [other wrong options] [Discuss in forum] 1-2 kb 40-44 kb 20-23 kb |
Q20 ⇒ Cos site of the cosmids both (a) and (b) [other wrong options] [Discuss in forum] consists of 12 bases helps whole genome in circularization and ligation contains cleavage site |
Or |