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Home⇒ Medical Science⇒ Biotechnology⇒ Media required for plant cell culture
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Which is the most common carbon source used in the plant cell culture media? Sucrose [other wrong options] [Discuss in forum] Glucose Fructose Maltose |
Q2 ⇒ Concentration of sucrose generally used in plant cell culture media is 20-30 g/l [other wrong options] [Discuss in forum] 10-15 g/l 40-50 g/l 60-70 g/l |
Q3 ⇒ What are the macronutrients used in plant cell culture medium? N, P, K, S, Ca [other wrong options] [Discuss in forum] N, P, K, S, Na N, P, K, Ca, Cl N, P, Ca, Na, Cl |
Q4 ⇒ Neutralized activated charcoal is occasionally added to young regenerating cultures to both (a) and (b) [other wrong options] [Discuss in forum] remove toxic phenolics produced by the stressed plant cell help to remove plants growth regulators introduced at an earlier stage maintain the pH of the medium |
Q5 ⇒ Which of the following growth regulator promote cell division? Cytokinins [other wrong options] [Discuss in forum] Auxins Gibberellins Brassinosteroids |
Q6 ⇒ Which of the following is not a cytokinin? 2,4-dichlorophenoxyacetic acid [other wrong options] [Discuss in forum] 6 benzylaminopurine Zeatin Kinetin |
Q7 ⇒ Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise [other wrong options] [Discuss in forum] utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise utilization of both ammonium and nitrate result in rise in pH utilization of both ammonium and nitrate result in drop in pH |
Q8 ⇒ Which is/are the disadvantage/(s) of using IAA in plant cell culture media? All of the above [other wrong options] [Discuss in forum] It is unstable in solution Gets easily oxidized Conjugated to inactive form by plant cells |
Q9 ⇒ In plant cell culture media, auxins and cytokinins are used in the range of l-50μM [other wrong options] [Discuss in forum] 50-100μM 100-125μM more than 125μM |
Q10 ⇒ What is 'nurse' or conditioned medium? It is the liquid medium removed from the suspension of fast growing cells [other wrong options] [Discuss in forum] It is the media full of growth factors used for the growth of cells It is the medium added to nurse the callus culture Both (a) and (b) |
Q11 ⇒ Which of the following growth regulator cause plant cells to grow? Auxins [other wrong options] [Discuss in forum] Cytokinins Gibberellins Brassinosteroids |
Q12 ⇒ Which of the following is not true about nurse or conditioned medium? it is removed aseptically from the culture and is autoclaved before use [other wrong options] [Discuss in forum] It is liquid removed from the suspension of fast growing cells It contains uncharacterized growth factor released by growing cells It is used in the culture of regenerating protoplast |
Q13 ⇒ Which of the following growth regulator is added for short initiation during plant regeneration from callus? Cytokinins [other wrong options] [Discuss in forum] Auxins Gibberellins Brassinosteroids |
Q14 ⇒ To maintain the pH of the culture both (a) and (b) [other wrong options] [Discuss in forum] organic acid such as citric, fumaric, malic and succinic acid is used synthetic buffers such as Tris, MES or HEPS are used ammonium salts are used |
Q15 ⇒ Which of the following is an ethylene biosynthesis inhibitor? Silver thiosulphate [other wrong options] [Discuss in forum] Citric acid Succinic acid Activated charcoal |
Q16 ⇒ Which is/are the naturally occurring plant auxins? Indole acetic acid (IAA) [other wrong options] [Discuss in forum] Naphthalenacetic acid (NAA) 2,4-dichlorophenoxyacetic acid All of the above |
Q17 ⇒ Silver thiosulphate is added to culture medium as it helps to prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. [other wrong options] [Discuss in forum] maintain the pH remove toxic phenolics from plant cells all of the above |
Q18 ⇒ Very high sugar concentration (40-100 g/l) have been used Both (a) and (b) [other wrong options] [Discuss in forum] in specialized secondary metabolite production to adjust the osmotic potential of the media in short term treatment for regeneration none of these |
Q19 ⇒ Which of the following growth regulator is used to stimulate embryo or shoot development? Gibberellins [other wrong options] [Discuss in forum] Auxins Cytokinins Brassinosteroids |
Q20 ⇒ Which of the following is not an auxin? zeatin [other wrong options] [Discuss in forum] Indole acetic acid (IAA) Naphthalenacetic acid (NAA) Indole butyric acid |