Question is ⇒ Single stranded vectors are useful, Options are ⇒ (A) for sequencing of cloned DNA, (B) for oligonucleotide directed mutagenesis, (C) for probe preparation, (D) all of the above, (E) , Leave your comments or Download question paper.

Question is ⇒ Single stranded vectors are useful, Options are ⇒ (A) for sequencing of cloned DNA, (B) for oligonucleotide directed mutagenesis, (C) for probe preparation, (D) all of the above, (E) , Leave your comments or Download question paper. Previous question Next question


Q1.	Single stranded vectors are useful
A.	for sequencing of cloned DNA [Wrong Answer]
B.	for oligonucleotide directed mutagenesis [Wrong Answer]
C.	for probe preparation [Wrong Answer]
D.	all of the above [Correct Answer]
View Answer 2 -3 Explanation:- Answer : D Discuss it below : !! OOPS Login [Click here] is required to post your answer/result Help other students, write article, leave your comments

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⇒ Phagemid vectors are
combination of plasmid and phage λ
combination of phages and cosmid
phages carrying properties of plasmids
all of the above

⇒ Phagemid consist of
plasmid vector carrying λ phage's cos site
plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying origin of replication of plasmid only

⇒ Which of the following is not true about phagemid?
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain
Contain λ att site
Can only be propagated as phage

⇒ λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
6-7 kb
1-2 kb
40-44 kb
20-23 kb

⇒ Select the wrong statement about plasmids?
It is extrachromosomal
It is double stranded
Its replication depends upon host cell
It is closed and circular DNA

⇒ EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
6-7 kb
15-25 kb
40-44 kb
1-2 kb

⇒ Maximum size of foreign DNA that can be inserted into an insertion vector is
35 kb
18 kb
50 kb
27 kb

⇒ PBR 322 has/have which of the following selection marker(s)?
Amp^r
Tet^r
Both (a) and (b)
Kan^r

⇒ Difference between λ gt 10 and λ gt 11 vectors is that
λ gt 11 is an expression vector
λ gt 10 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is a replacement vector

⇒ P1 cloning vector is the example of
plasmid
cosmid
bacteriophage
phagemid

⇒ Inserted DNA in λ gt 11 can be expressed as
β-galactosidase fused protein
free protein in the cytoplasm
free protein that is secreted out
all of the above

⇒ Stuffer is
the right arm of the vector DNA
the left arm of the vector DNA
central fragment of the vector DNA
none of the above

⇒ Maximum size of foreign DNA that can be inserted into a replacement vector is
25-30 kb
18-20 kb
20-25 kb
40-50 kb

⇒ Cos site of the cosmids
consists of 12 bases
helps whole genome in circularization and ligation
both (a) and (b)
contains cleavage site

⇒ Cosmid vectors are used for
cloning small fragments of DNA
cloning large fragments of DNA
cloning prokaryotic DNA only
cloning eukaryotic DNA only

⇒ Plasmids which are maintained as multiple copy number per cell are known as
stringent plasmids
relaxed plasmids
cryptic plasmids
none of these

⇒ Cryptic plasmids
do not exhibit any phenotypic trait
exhibit many phenotypic traits
exhibit one phenotypic traits
exhibit antibiotic resistance

⇒ Conjugative plasmids
exhibit antibiotic resistance
do not exhibit antibiotic resistance
carry transfer genes called the tra genes
do not carry transfer genes

⇒ Cosmids lack
genes coding for viral proteins
origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA

⇒ Plasmids which are maintained as limited number of copies per cell are known as
stringent plasmids
relaxed plasmids
cryptic plasmids
all of these

⇒ Plasmid incompatibility is
inability of a plasmid to grow in the host
inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
both (a) and (b)
none of the above

⇒ Charon vectors are different from EMBL vectors because
they have more extensive range of restriction targets with in their polylinkers.
physical separation of lambda arm from central fragment is required
both (a) and (b)
physical separation of lambda arm from central fragment is not required

⇒ λ ZAP vector is an example of
phage
phagemid
cosmid
plasmid

⇒ Single stranded vectors are useful
for sequencing of cloned DNA
for oligonucleotide directed mutagenesis
for probe preparation
all of the above

⇒ Charon 34 and Charon 35 are the examples of
plasmid vector
cosmid vector
phage vector
phagemid vector

⇒ M 13 is an example of
filamentous phage
single stranded DNA vector
both (a) and (b)
plasmid

⇒ Size of the DNA that can be packaged into a λ phage is
50 kb
35-53 kb
40-50 kb
any size

⇒ Cosmid vectors are
plasmids that contain fragment of λ DNA including the cos site
phages that lack cos site
plasmids that have no selection marker
cryptic plasmids

⇒ A plasmid can be considered as a suitable cloning vector if
it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties
All of the above

⇒ Charon 34 and Charon 35 can clone DNA upto
1-2 kb
6-7 kb
9-20 kb
30-35 kb