Home⇒ Medical Science⇒ Biotechnology⇒ Recombinant dna
| 1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
| 2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
| Q1 ⇒ In genetic engineering, a chimera is a plasmid that contains foreign DNA [other wrong options] [Discuss in forum] |
| Q2 ⇒ Which of the following vector can maintain the largest fragment of foreign DNA? YAC [other wrong options] [Discuss in forum] |
| Q3 ⇒ The advantage of using DNA polymerases from thermophilic organisms in PCR is that the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands [other wrong options] [Discuss in forum] |
| Q4 ⇒ Vectors are molecules that are able to covalently bond to and carry foreign DNA into cells [other wrong options] [Discuss in forum] |
| Q5 ⇒ Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid? DNA ligase [other wrong options] [Discuss in forum] |
| Q6 ⇒ Electroporation is the application of high voltage pulses [other wrong options] [Discuss in forum] |
| Q7 ⇒ For gene probes to be useful they must both (a) and (b) [other wrong options] [Discuss in forum] |
| Q8 ⇒ Agrobacterium tumefaciens is a bacterium that can be used to introduce DNA into plants [other wrong options] [Discuss in forum] |
| Q9 ⇒ Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are restriction endonucleases [other wrong options] [Discuss in forum] |
| Q10 ⇒ An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as a transgenic animal [other wrong options] [Discuss in forum] |
| Q11 ⇒ The Southern blotting technique depends on similarities between the sequences of probe DNA and experimental DNA [other wrong options] [Discuss in forum] |
| Q12 ⇒ Which of the following is not commonly used as vector? Fungi [other wrong options] [Discuss in forum] |
| Q13 ⇒ Which type of restriction endonuclease cuts the DNA within the recognition site? Type II [other wrong options] [Discuss in forum] |
| Q14 ⇒ Which of the following is obtained using processed mRNA molecules as a template? cDNA [other wrong options] [Discuss in forum] |
| Q15 ⇒ A genomic library is a collection of many clones possessing different DNA fragments from the same organisms bound to vectors [other wrong options] [Discuss in forum] |
| Q16 ⇒ Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using expression vectors [other wrong options] [Discuss in forum] |
| Q17 ⇒ A short molecule containing 2-20 nucleotide is oligonucleotide [other wrong options] [Discuss in forum] |
| Q18 ⇒ The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of genetic engineering [other wrong options] [Discuss in forum] |
| Q19 ⇒ The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as gene gun [other wrong options] [Discuss in forum] |
| Q20 ⇒ Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as gel electrophoresis [other wrong options] [Discuss in forum] |
| Or |