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Home⇒ Medical Science⇒ Biotechnology⇒ Gene transfer in plants
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Which of the following is not true about the helper plasmids? These can replicate in Agrobacterium [other wrong options] [Discuss in forum] These help in the mediating conjugation of intermediate vectors These can't replicate in Agrobacterium All of the above |
Q2 ⇒ In the liposome mediated gene transfer in plants, nucleic acids are both (a) and (b) [other wrong options] [Discuss in forum] protected from nuclease digestion stable in liposomes not stable in liposomes |
Q3 ⇒ Opines that are present in crown gall tumour include all of these [other wrong options] [Discuss in forum] octopine nopaline agropine |
Q4 ⇒ Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast? It employs needle with diameter greater than cell diameter [other wrong options] [Discuss in forum] It is carried out with the help of micromanipulator The recipient cells are immobilized on artificial support or artificially bound to substarate all of the above |
Q5 ⇒ Microinjection involves injection with needle having diameter greater than cell diameter [other wrong options] [Discuss in forum] injection of large amount of DNA injection of DNA into bigger cells all of the above |
Q6 ⇒ Co-integrating transformation vectors must include a region of homology in between vector plasmid and Ti-plasmid [other wrong options] [Discuss in forum] the vector plasmid the Ti-plasmid none of these |
Q7 ⇒ Microprojectile method of gene transfer in plants involves delivery of DNA with the help of bolistics [other wrong options] [Discuss in forum] with the help of micromanipulator with the help of needles any of the above |
Q8 ⇒ The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as border sequences [other wrong options] [Discuss in forum] cos site flanking sequences transfer sequences |
Q9 ⇒ Direct DNA uptake by protoplasts can be stimulated by polyethylene glycol (PEG) [other wrong options] [Discuss in forum] decanal luciferin all of these |
Q10 ⇒ On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of 25 bp [other wrong options] [Discuss in forum] 12 bp 20 bp 30 bp |
Q11 ⇒ Opine synthesis is the property both (a) and (b) [other wrong options] [Discuss in forum] conferred to plant cells when it transformed by Agrobacterium tumefaciens determined by the bacteria Agrobacterium tumefaciens of normal plant cells |
Q12 ⇒ Vir genes required for the T-DNA transfer and processing are located outside the T-DNA region [other wrong options] [Discuss in forum] on the T-DNA on the plant genome none of these |
Q13 ⇒ The size of the virulent plasmid of Agrobacterium tumefaciens is 140-235 kb [other wrong options] [Discuss in forum] 40-80 kb 80-120 kb >235 kb |
Q14 ⇒ Which of the following is true about Agrobacterium tumefaciens? All of the above [other wrong options] [Discuss in forum] It causes crown gall disease of plants It infects gymosperms It infects dicotyledonous angiosperms |
Q15 ⇒ Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco? Both (a) and (b) [other wrong options] [Discuss in forum] Acetosyringone α-hydroxy syringone None of these |
Q16 ⇒ The left segment of octopine T-DNA (TL) is necessary for tumour formation [other wrong options] [Discuss in forum] enzymes for agropine biosynthesis conjugative transfer all of these |
Q17 ⇒ In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by vir D [other wrong options] [Discuss in forum] vir A vir B vir C |
Q18 ⇒ Integrated nopaline T-DNA occurs as single segment [other wrong options] [Discuss in forum] two segments three segments four segments |
Q19 ⇒ Integrated octopine T DNA occurs as two segments [other wrong options] [Discuss in forum] single segment three segments four segments |
Q20 ⇒ Which of the following is true about T DNA? Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA [other wrong options] [Discuss in forum] Integration of T DNA occurs only at one specific sites in the plant nuclear DNA Integration of T DNA occurs at two specific sites in the plant nuclear DNA Integration of T DNA occurs at one site that may be random in the plant nuclear DNA |
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