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Home⇒ Medical Science⇒ Biotechnology⇒ Animal cell culture and regulation
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Which of the following is incorrect? ECL grow in higher density [other wrong options] [Discuss in forum] Established cell lines (ECL) have short doubling time ECL are invariably aneuploid ECL do not show much evidence of spatial orientation |
Q2 ⇒ What are the main constituents of culture for animal cell growth? Glucose and Glutamine [other wrong options] [Discuss in forum] Growth factors Cytokines All of these |
Q3 ⇒ Cells which have undergone transformation frequently become anchorage independent [other wrong options] [Discuss in forum] anchorage dependent stable unstable |
Q4 ⇒ Which of the following is the technique used for the embryo culture? All of these [other wrong options] [Discuss in forum] Organ cultures on plasma clots Organ cultures on agar Whole embryo cultures |
Q5 ⇒ Disaggregating of cells can be achieved by all of the above [other wrong options] [Discuss in forum] physical disruption enzymatic digestion treating with chelating agents |
Q6 ⇒ pH of culture medium is initially controlled by presence of bicarbonate buffer [other wrong options] [Discuss in forum] presence of CO2 addition of bases none of these |
Q7 ⇒ Toxicity due to accumulation of ammonia can be overcome all of the above [other wrong options] [Discuss in forum] by substituting glutamine by glutamate by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium |
Q8 ⇒ An established cell line can be called where it has been subcultured at least 70 times at an interval of 3 days between subcultures [other wrong options] [Discuss in forum] 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures |
Q9 ⇒ Range of optimum glutamine concentration present in the culture media is 2-7 mmol/litre [other wrong options] [Discuss in forum] 1-2 mmol/litre 7-15 mmol/litre 15 - 20 mmol/litre |
Q10 ⇒ The technique of organ culture may be divided on the basis of employing both (a) and (b) [other wrong options] [Discuss in forum] solid medium liquid medium semi-solid medium |
Q11 ⇒ Excess CO2 suppress cell growth and productivity by both (a) and (b) [other wrong options] [Discuss in forum] inhibiting respiration altering intracellular pH by diffusing across cell membrane altering pH of the medium |
Q12 ⇒ To prevent the accumulation of lactate low glucose concentration is required [other wrong options] [Discuss in forum] low glutamine concentration is required high glutamine concentration is required high glucose concentration is required |
Q13 ⇒ Which of the following is not the explantation technique? Adherent primary culture [other wrong options] [Discuss in forum] Slide culture Carrel flask culture Roller test tube culture |
Q14 ⇒ Higher dissolved oxygen concentration in the culture media are toxic and lead to all of the above [other wrong options] [Discuss in forum] DNA degradation lipid peroxidation metabolism of nutrients in culture media at a rate greater than that required for consumption |
Q15 ⇒ When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) [other wrong options] [Discuss in forum] incomplete glutamine oxidation increase in specific lactate production from glucose accumulation of ammonia |
Q16 ⇒ Which of the following is correct? ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL) [other wrong options] [Discuss in forum] ECL and PCL can be established in suspension cultures PCL can be established in suspension cultures none of the above |
Q17 ⇒ In animal cell culture, particularly mammalian cell culture, transformation means phenotypic modifications of cells in culture [other wrong options] [Discuss in forum] uptake of new genetic material both (a) and (b) release of genetic information |
Q18 ⇒ Which of the following is not a source of energy in active muscle cells? Lactic acid [other wrong options] [Discuss in forum] Creatine phosphate ATP Glucose |
Q19 ⇒ Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 280-360 [other wrong options] [Discuss in forum] 150-300 300-325 360-400 |
Q20 ⇒ The human fibroplast is a classical example of stable primary cell lines [other wrong options] [Discuss in forum] established cell lines cell transformations none of these |
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