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Home⇒ Medical Science⇒ Biotechnology⇒ Vectors uses for animal cell culture
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ Which of the following histone is not present in SV40 virus? H1 [other wrong options] [Discuss in forum] H2A H3 H4 |
Q2 ⇒ SV 40 vectors, in which early region replacements are carried out, require COS cell line [other wrong options] [Discuss in forum] SV 40 helper virus pBR 322 vector none of these |
Q3 ⇒ Which one of the following is true about Yeast Integrative plasmids (YIp) vectors? Allows transformation by using crossing over [other wrong options] [Discuss in forum] Has a origin of replication and rep genes Carries autonomously replicating sequence Carries centromere and telomere sequences |
Q4 ⇒ Which of the following are produced in high copy number? Both (a) and (b) [other wrong options] [Discuss in forum] YEp YRp YCp |
Q5 ⇒ Yeast Replicating plasmids (YRp) carry autonomously replicating sequence [other wrong options] [Discuss in forum] centromere telomere both (a) and (c) |
Q6 ⇒ Yeast episomal plasmids (YEp) were constructed using 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9 [other wrong options] [Discuss in forum] 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322 12μm yeast plasmid and E coli vector pMB 9 12μm yeast plasmid and E coli vector pBR 322 |
Q7 ⇒ Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of Baculo virus [other wrong options] [Discuss in forum] Vaccinia virus Papilloma virus Retrovirus |
Q8 ⇒ Bovine papilloma virus has a circular double stranded DNA genome of about 7.9 kb [other wrong options] [Discuss in forum] 2.5 kb 5.2 kb 9.5 kb |
Q9 ⇒ In case of Baculoviruses, the foreign gene is inserted between the polyhedrin promoter and downstream sequences [other wrong options] [Discuss in forum] is inserted downstream of polyhedrin promoter is inserted downstream of viral origin of replication is inserted randomly in viral genome |
Q10 ⇒ Which one of the following is true about Yeast Episomal plasmids (YEp) vectors? Has a origin of replication and rep genes [other wrong options] [Discuss in forum] Allows transformation by using crossing over Carries autonomously replicating sequence Carries centromere and telomere sequences |
Q11 ⇒ Which of the following promoters is repressed in presence of glucose? Galactokinase (GAL 1) [other wrong options] [Discuss in forum] Acid phosphatase (PHOS) Both (a) and (b) Phosphoglycerate kinase (PGK) |
Q12 ⇒ Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA either episomally or integrated at high copy number [other wrong options] [Discuss in forum] episomally only integrated only either episomally or integrated at low copy number |
Q13 ⇒ Which one of the following is the most stable vector? YAC [other wrong options] [Discuss in forum] YEp YRp YIp |
Q14 ⇒ Moloney-Mu-leukemia virus is an example of Retro virus [other wrong options] [Discuss in forum] Vaccinia virus Baculo virus none of these |
Q15 ⇒ Which of the following(s) is/are the most commonly used markers in yeast? All of these [other wrong options] [Discuss in forum] His 3 Ura 3 Trp 1 |
Q16 ⇒ Recombinant adeno virus can be created by deleting E1A/E 1B genes that are transcriptional regulators and are essential for viral replication [other wrong options] [Discuss in forum] not necessary for viral replication coding for virus coat protein important for packaging of viral DNA into viral head |
Q17 ⇒ Maximum amount of foreign DNA introduced into SV 40 viruses is 2.5 kb [other wrong options] [Discuss in forum] 10 kb 5 kb 1 kb |
Q18 ⇒ Cos cell line is derivative of permissive CV-1 monkey cell line [other wrong options] [Discuss in forum] cohesive end site of phage λ cohesive initial site of phage λ derivative of non-permissive CV-1 monkey cell line |
Q19 ⇒ Maximum insert size in case of adenovirus vectors is 6-8 kb [other wrong options] [Discuss in forum] 4-6 kb 10-12 kb 20 kb |
Q20 ⇒ α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is Lys 2 [other wrong options] [Discuss in forum] His 3 Ura 3 Trp 1 |
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