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Home⇒ Medical Science⇒ Biotechnology⇒ Gene transfer in plants
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q21 ⇒ Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into pBR 322 based plasmid vector [other wrong options] [Discuss in forum] pRK 2013 pRN 3 all of these |
Q22 ⇒ In a plant tumour cell only a small specific segment of callus T DNA is incorporated [other wrong options] [Discuss in forum] complete Ti plasmid is incorporated in plant nuclear DNA different parts of the Ti-plasmid are incorporated may vary from plant to plant |
Q23 ⇒ Agrobacterium tumefaciens is a gram (-) bacteria [other wrong options] [Discuss in forum] gram (+) bacteria a fungi a yeast |
Q24 ⇒ Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain two sets of sequence necessary for gene transfer [other wrong options] [Discuss in forum] one set of sequence necessary for gene transfer three sets of sequence necessary for gene transfer four sets of sequence necessary for gene transfer |
Q25 ⇒ Plant transformation vectors based on Agrobacterium can generally be divided into two vectors [other wrong options] [Discuss in forum] four vectors six vectors eight vectors |
Q26 ⇒ Which technique is used to introduce genes into dicots? Ti plasmid infection [other wrong options] [Discuss in forum] electroporation particle acceleration microinjection |
Q27 ⇒ Virulence trait of Agrobacterium tumefaciens is borne on tumour inducing plasmid DNA [other wrong options] [Discuss in forum] chromosomal DNA both chromosomal and plasmid DNA cryptic plasmid DNA |
Q28 ⇒ Advantage of microprojectile method over microinjection method for gene transfer in plants include all of the above [other wrong options] [Discuss in forum] intact cells are used method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall gene can be transferred to many cells simultaneously |
Q29 ⇒ Opines are amino acid derivatives found in tumor tissues [other wrong options] [Discuss in forum] amino acid derivatives found in normal tissues amino acid derivatives found in both normal as well as tumor tissues none of the above |
Q30 ⇒ The right segment of octopine T-DNA (TR) is necessary for enzymes for agropine biosynthesis [other wrong options] [Discuss in forum] tumour formation conjugative transfer all of these |
Q31 ⇒ Liposomes mediated gene transfer in plants involves all of the above [other wrong options] [Discuss in forum] plasmid DNA enclosed in a lipid bag fusion of liposomes with protoplast use of polyethylene glycol (PEG) |
Q32 ⇒ Which of the following are used as selection marker for the cells transformed with Agrobacterium? any of the above [other wrong options] [Discuss in forum] Neomycin phosphotransferase Streptomycin phosphotransferase Hygromycin phosphotransferase |
Q33 ⇒ Which of the following is not true about the direct repeats flanking T-DNA? These repeats are transferred intact to the plant genome [other wrong options] [Discuss in forum] They are conserved between nopaline and octopine Ti-plasmids These are important in integration mechanism all of the above |
Q34 ⇒ The transfer of intermediate vectors into Agrobacterium are brought about by triparental mating [other wrong options] [Discuss in forum] transformation biparental mating transduction |
Q35 ⇒ Crown gall tissue both (a) and (b) [other wrong options] [Discuss in forum] can be cultivated in vitro in absence of bacteria retains tumorous properties when cultivated shows tumorous properties only in presence of bacteria |
Q36 ⇒ Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes? vir A and vir G [other wrong options] [Discuss in forum] vir C and vir D vir B and vir E vir A and vir B |
Q37 ⇒ Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid? either (a) or (b) [other wrong options] [Discuss in forum] pRK 2013 pRN 3 none of these |
Q38 ⇒ T-DNA transfer and processing into plant genome requires products of which of the following genes? All of these [other wrong options] [Discuss in forum] vir A,B vir G,C vir D,E |
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