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Home⇒ Medical Science⇒ Bochemical Engineering⇒ Immobilized enzyme
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ For a steady state condition, the change of substrate concentration (dCs/dt) is zero [other wrong options] [Discuss in forum] 1 >1 <1 |
Q2 ⇒ Which of the following is the commonly employed adsorbents? All of these [other wrong options] [Discuss in forum] Calcium carbonate Alumina Celluloses |
Q3 ⇒ Which of the following is considered as a disadvantage to the adsorption method of immobilization? State of immobilization is very sensitive to solution pH, ionic strength and temperature [other wrong options] [Discuss in forum] It is possible to separate and purify the enzymes while being immobilized The enzymes are not usually deactivated by adsorption The adsorption is a reversible process |
Q4 ⇒ Commonly employed water insoluble supports for the covalent attachment of enzyme include all of these [other wrong options] [Discuss in forum] acrylamide based polymers polypeptides dextran |
Q5 ⇒ To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as actual reaction rate/ rate if not slowed by diffusion [other wrong options] [Discuss in forum] reaction rate/rate if not slowed by diffusion rate if not slowed by diffusion/ reaction rate rate if not slowed by diffusion/ actual reaction rate |
Q6 ⇒ Which of the following is taken as an assumption in the distributed model? All of the above [other wrong options] [Discuss in forum] The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme. Mass transfer through the immobilized enzyme occurs via molecular diffusion There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme |
Q7 ⇒ The most commonly employed cross-linked polymer is the polyacrylamide gel [other wrong options] [Discuss in forum] collagen celluloses cation exchange resin |
Q8 ⇒ The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by copolymerization or microencapsulation [other wrong options] [Discuss in forum] crosslinking using multifunctional reagents adsorption all of the above |
Q9 ⇒ The immobilized enzyme produced by micro encapsulation technique provides an extremely large surface area [other wrong options] [Discuss in forum] smaller surface area high amount of solvent relatively smaller surface area |
Q10 ⇒ Which of the following is not the correct answer? None of the above [other wrong options] [Discuss in forum] Immobilized enzyme may show selectively altered chemical properties Immobilized enzyme may show selectively altered physical properties Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell |
Q11 ⇒ The covalent attachment of enzyme molecules is via nonessential amino acids residues to water insoluble, functional supports [other wrong options] [Discuss in forum] essential amino acids residues to water insoluble, functional supports nonessential amino acids residues to water soluble, functional supports essential amino acids residues to water soluble, functional supports |
Q12 ⇒ Which medium is used for the production of Penicillin using immobilized cells glucose medium [other wrong options] [Discuss in forum] 1% peptone medium Yeast extract medium LB broth |
Q13 ⇒ Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have low molecular weight [other wrong options] [Discuss in forum] high molecular weight high equivalent weight low reactivity |
Q14 ⇒ If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles) rp = ksaCsb [other wrong options] [Discuss in forum] rp = ksa(Csb-Cs) rp = ksaCs rp = ksa(Csb+Cs) |
Q15 ⇒ Damkohler number(NDa) is the ratio of the maximum reaction rate to the maximum mass transfer rate [other wrong options] [Discuss in forum] the ratio of the minimum reaction rate to the maximum mass transfer rate the ratio of the maximum reaction rate to the minimum mass transfer rate the ratio of the minimum reaction rate to the minimum mass transfer rate |
Q16 ⇒ The immobilized technique involving chemical method is covalent bond formation dependent [other wrong options] [Discuss in forum] non-covalent bond formation dependent both (a) and (b) ionic bond formation dependent |
Q17 ⇒ Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses a diffusion cell with two compartments divided by a thin gel [other wrong options] [Discuss in forum] a diffusion cell with two compartments a diffusion cell with two compartments plus by a thin gel a diffusion cell with two compartments multiply by a thin gel |
Q18 ⇒ The support material for immobilization of cells of Bacillus subtilis is agarose and carbodiimide [other wrong options] [Discuss in forum] ion exchange resins gelatin Anthracite |
Q19 ⇒ The immobilization technique involving physical method is non-covalent bond formation dependent [other wrong options] [Discuss in forum] covalent bond formation dependent both (a) and (b) ionic bond formation dependent |
Q20 ⇒ During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is equal to that of substrate consumption [other wrong options] [Discuss in forum] more than that of substrate consumption lesser than that of substrate consumption is nothing to do with the substrate consumption |
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