@⇒ Polymerase chain reaction ⇒ Microbiology ⇒ Medical Science Engineering Multiple Choice Questions / Objective type questions, MCQ's, with question and answers, download free PDF, short cut notes, rapid fire notes, best theory

Home⇒ Medical Science⇒ Microbiology⇒ Polymerase chain reaction

Note:- You can add tough questions into your favourite list for second revision.


Q1.	The efficiency of amplification in PCR in later cycles is reduced due to
A.	reduction in substrate concentration [Wrong Answer]
B.	insufficient enzyme and time to synthesize mass quantity of DNA [Wrong Answer]
C.	build up of PCR product which competes with primers for hybrid formation [Wrong Answer]
D.	all of these [Correct Answer]
View Answer 2 -1 Explanation:- Answer : D Discuss it below : Click here for more comments


Q2.	The template for PCR is
A.	RNA [Wrong Answer]
B.	single stranded DNA [Wrong Answer]
C.	double stranded DNA [Correct Answer]
D.	none of these [Wrong Answer]
View Answer 1 -1 Explanation:- Answer : C Discuss it below : Click here for more comments


Q3.	The PCR/sequencing approach to rRNA is most likely to give information about
A.	the species to which the bacteria are most closely related [Correct Answer]
B.	morphology of the bacterium [Wrong Answer]
C.	the type of energy metabolism the bacterium has [Wrong Answer]
D.	whether the bacterium is motile [Wrong Answer]
View Answer 2 -1 Explanation:- Answer : A Discuss it below : Click here for more comments


Q4.	The cloning step in PCR/sequencing analysis of microbial communities is necessary for
A.	the amplification process [Wrong Answer]
B.	preventing contamination by outside DNA [Wrong Answer]
C.	separating the different rRNA gene sequences in the mixture [Correct Answer]
D.	none of these [Wrong Answer]
View Answer 1 -1 Explanation:- Answer : C Discuss it below : Click here for more comments


Q5.	The original enzyme(s) used in PCR reaction is/are known as
A.	E. coli DNA polymerase [Correct Answer]
B.	E. coli RNA polymerase [Wrong Answer]
C.	Taq polymerase [Wrong Answer]
D.	all of these [Wrong Answer]
View Answer 1 -7 Explanation:- Answer : A Discuss it below : Click here for more comments

1 2 ..Next.>> Page 1 of 2

Or