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Home⇒ Medical Science⇒ Microbiology⇒ Polymerase chain reaction
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q1 ⇒ The efficiency of amplification in PCR in later cycles is reduced due to all of these [other wrong options] [Discuss in forum] ![]() ![]() reduction in substrate concentration insufficient enzyme and time to synthesize mass quantity of DNA build up of PCR product which competes with primers for hybrid formation |
Q2 ⇒ The template for PCR is double stranded DNA [other wrong options] [Discuss in forum] ![]() ![]() RNA single stranded DNA none of these |
Q3 ⇒ The PCR/sequencing approach to rRNA is most likely to give information about the species to which the bacteria are most closely related [other wrong options] [Discuss in forum] ![]() ![]() morphology of the bacterium the type of energy metabolism the bacterium has whether the bacterium is motile |
Q4 ⇒ The cloning step in PCR/sequencing analysis of microbial communities is necessary for separating the different rRNA gene sequences in the mixture [other wrong options] [Discuss in forum] ![]() ![]() the amplification process preventing contamination by outside DNA none of these |
Q5 ⇒ The original enzyme(s) used in PCR reaction is/are known as E. coli DNA polymerase [other wrong options] [Discuss in forum] ![]() ![]() E. coli RNA polymerase Taq polymerase all of these |
Q6 ⇒ Polymerase chain reaction basically consists of three steps [other wrong options] [Discuss in forum] ![]() ![]() two steps four steps five steps |
Q7 ⇒ Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? All of these [other wrong options] [Discuss in forum] ![]() ![]() HIV-1 and HIV-2 viruses Hepatitis B virus Mycobacterium tuberculosis |
Q8 ⇒ RNA populations can also be used as PCR templates after reverse transcription into c DNA [other wrong options] [Discuss in forum] ![]() ![]() t RNA m DNA r RNA |