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Home⇒ Medical Science⇒ Biochemistry⇒ Recombinant dna technology
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q21 ⇒ Which of these genes codes for a protein that plays a role in white blood cell function? MPO [other wrong options] [Discuss in forum] DCP1 GLUT4 RP13 |
Q22 ⇒ The TP53 gene of chromosome 17 codes for a protein __________ . involved in the regulation of the cell cycle [other wrong options] [Discuss in forum] that plays a role in the digestive process involved in glucose transport that is like a white blood cell protein |
Q23 ⇒ The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends [other wrong options] [Discuss in forum] single strands restriction fragments ligases |
Q24 ⇒ Restriction enzymes all of the above [other wrong options] [Discuss in forum] protect bacteria from viral infection cut DNA in a staggered fashion cut DNAs producing a blunt end |
Q25 ⇒ Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are short sequences that will hybridize only to a specific base sequence [other wrong options] [Discuss in forum] copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) |
Q26 ⇒ Knockout mice are created by transfecting embryonic stem cells with an altered gene sequence [other wrong options] [Discuss in forum] mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus |
Q27 ⇒ A human cDNA library contains DNA for virtually all of the human proteins in vectors [other wrong options] [Discuss in forum] contains DNA for specific human proteins cannot be used to obtain human genes because it would be radioactive none of the above |
Q28 ⇒ Restriction enzymes are named for the bacterium they are derived from [other wrong options] [Discuss in forum] the person who discovered the viral DNA that they attack none of the above |
Q29 ⇒ Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because all of the above [other wrong options] [Discuss in forum] in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio both techniques utilize the sieving properties of gels |
Q30 ⇒ The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase [other wrong options] [Discuss in forum] treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase |
Q31 ⇒ An expression vector all of the above [other wrong options] [Discuss in forum] always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host always contains DNA segments for the regulation of mRNA production |
Q32 ⇒ In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. negative ... positive [other wrong options] [Discuss in forum] basic ... acidic long ... short positive to negative |
Q33 ⇒ An example of a restriction fragment length polymorphism is different length fragments of DNA resulting from loss or gain of a restriction site [other wrong options] [Discuss in forum] an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved all of the above |
Q34 ⇒ Which of the following pair will produce complementary sticky ends? Sau 3A & Bam HI [other wrong options] [Discuss in forum] Eco RI & MspI Msp I & HPA II Mbo I & Sau 3A |
Or |