Question is ⇒ In animal cell culture, particularly mammalian cell culture, transformation means, Options are ⇒ (A) uptake of new genetic material, (B) phenotypic modifications of cells in culture, (C) both (a) and (b), (D) release of genetic information, (E) , Leave your comments or Download question paper.

Question is ⇒ In animal cell culture, particularly mammalian cell culture, transformation means, Options are ⇒ (A) uptake of new genetic material, (B) phenotypic modifications of cells in culture, (C) both (a) and (b), (D) release of genetic information, (E) , Leave your comments or Download question paper. Previous question Next question


Q1.	In animal cell culture, particularly mammalian cell culture, transformation means
A.	uptake of new genetic material [Wrong Answer]
B.	phenotypic modifications of cells in culture [Correct Answer]
C.	both (a) and (b) [Wrong Answer]
D.	release of genetic information [Wrong Answer]
View Answer 2 -1 Explanation:- Answer : B Discuss it below : !! OOPS Login [Click here] is required to post your answer/result Help other students, write article, leave your comments

Previous question Next question

Also Read Similar Questions Below :

⇒ In animal cell culture, particularly mammalian cell culture, transformation means
uptake of new genetic material
phenotypic modifications of cells in culture
both (a) and (b)
release of genetic information

⇒ How does CO₂ help in the cell metabolism during cell culture?
It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
For monitoring pH of the culture
All of the above

⇒ Cells which have undergone transformation frequently become
anchorage independent
anchorage dependent
stable
unstable

⇒ Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
Down's syndrome
Turner's syndrome
Klinefelter's syndrome
all of these

⇒ Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
established cell lines
primary cell lines
secondary cell lines
propagated cell lines

⇒ According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
6 amino acids
8 amino acids
10 amino acids
12 amino acids

⇒ The human fibroplast is a classical example of
stable primary cell lines
established cell lines
cell transformations
none of these

⇒ Higher dissolved oxygen concentration in the culture media are toxic and lead to
DNA degradation
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
all of the above

⇒ For culturing, plasma from the adult chicken is preferred to mammalian plasma because
it forms a clear and solid coagulum even after dilution
it is too opaque
it doesn't produce solid clots
it forms a semi solid coagulum

⇒ What is the concentration of CO₂ required for culturing animal cells?
2-5%
1-10%
10-15%
15-20%

⇒ During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Ethyl alcohol is being produced in excess
The cells have too much oxygen
Glycolysis is being inhibited
The cells do not have enough oxygen

⇒ Excess CO₂ suppress cell growth and productivity by
inhibiting respiration
altering intracellular pH by diffusing across cell membrane
both (a) and (b)
altering pH of the medium

⇒ When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)
accumulation of ammonia

⇒ PH of culture medium is initially controlled by
presence of CO₂
presence of bicarbonate buffer
addition of bases
none of these

⇒ Toxicity due to accumulation of ammonia can be overcome
by substituting glutamine by glutamate
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
all of the above

⇒ At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
oxidative pathway
anaerobic pathway
both (a) and (b)
none of these

⇒ Which of the following is the technique used for the embryo culture?
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures
All of these

⇒ Which of the following is not the explantation technique?
Slide culture
Carrel flask culture
Roller test tube culture
Adherent primary culture

⇒ Which of the following is not a source of energy in active muscle cells?
Creatine phosphate
ATP
Lactic acid
Glucose

⇒ Range of osmolarity tolerated/accepted in mOsm/Kg of H₂O by mammalian cells is
150-300
280-360
300-325
360-400

⇒ What are the main constituents of culture for animal cell growth?
Glucose and Glutamine
Growth factors
Cytokines
All of these

⇒ Which of the following is incorrect?
Established cell lines (ECL) have short doubling time
ECL are invariably aneuploid
ECL grow in higher density
ECL do not show much evidence of spatial orientation

⇒ Which of the followings are the metabolic products of glucose and glutamine?
CO₂ and NH₃
CO₂ and lactate
Lactate and ammonium
Lactate only

⇒ Accumulation of lactate leads to
increase in pH
no change in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability

⇒ Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
0.05-5 nmol of O₂/cell/h
5 - 10 nmol of O₂ /cell/h
0.05-5 pmol of O₂/cell/h
5 - 10 pmol of O₂/cell/h

⇒ To prevent the accumulation of lactate
low glutamine concentration is required
high glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

⇒ Disaggregating of cells can be achieved by
physical disruption
enzymatic digestion
treating with chelating agents
all of the above

⇒ An established cell line can be called where it has been subcultured at least
70 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
50 times at an interval of 3 days between subcultures

⇒ What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
They act as growth promoters
They act as growth inhibitors
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth

⇒ The technique of organ culture may be divided on the basis of employing
solid medium
liquid medium
both (a) and (b)
semi-solid medium