Question is ⇒ Charon vectors are different from EMBL vectors because, Options are ⇒ (A) they have more extensive range of restriction targets with in their polylinkers., (B) physical separation of lambda arm from central fragment is required, (C) both (a) and (b), (D) physical separation of lambda arm from central fragment is not required, (E) , Leave your comments or Download question paper.

Question is ⇒ Charon vectors are different from EMBL vectors because, Options are ⇒ (A) they have more extensive range of restriction targets with in their polylinkers., (B) physical separation of lambda arm from central fragment is required, (C) both (a) and (b), (D) physical separation of lambda arm from central fragment is not required, (E) , Leave your comments or Download question paper. Previous question Next question


Q1.	Charon vectors are different from EMBL vectors because
A.	they have more extensive range of restriction targets with in their polylinkers. [Wrong Answer]
B.	physical separation of lambda arm from central fragment is required [Wrong Answer]
C.	both (a) and (b) [Correct Answer]
D.	physical separation of lambda arm from central fragment is not required [Wrong Answer]
View Answer 2 -2 Explanation:- Answer : C Discuss it below : !! OOPS Login [Click here] is required to post your answer/result Help other students, write article, leave your comments

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⇒ Select the wrong statement about plasmids?
It is extrachromosomal
It is double stranded
Its replication depends upon host cell
It is closed and circular DNA

⇒ Maximum size of foreign DNA that can be inserted into a replacement vector is
25-30 kb
18-20 kb
20-25 kb
40-50 kb

⇒ P1 cloning vector allow cloning of DNA of the length of
100 kbp
50 kbp
20 kbp
10 kbp

⇒ Charon 34 and Charon 35 are the examples of
plasmid vector
cosmid vector
phage vector
phagemid vector

⇒ EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
6-7 kb
15-25 kb
40-44 kb
1-2 kb

⇒ Difference between λ gt 10 and λ gt 11 vectors is that
λ gt 11 is an expression vector
λ gt 10 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is a replacement vector

⇒ Plasmids which are maintained as multiple copy number per cell are known as
stringent plasmids
relaxed plasmids
cryptic plasmids
none of these

⇒ Charon 34 and Charon 35 can clone DNA upto
1-2 kb
6-7 kb
9-20 kb
30-35 kb

⇒ λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
6-7 kb
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⇒ Which of the following is not true about phagemid?
Contain functional origin of replication of the plasmid and λ phage
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Contain λ att site
Can only be propagated as phage

⇒ Conjugative plasmids
exhibit antibiotic resistance
do not exhibit antibiotic resistance
carry transfer genes called the tra genes
do not carry transfer genes

⇒ Cosmid vectors are used for
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cloning large fragments of DNA
cloning prokaryotic DNA only
cloning eukaryotic DNA only

⇒ Plasmids which are maintained as limited number of copies per cell are known as
stringent plasmids
relaxed plasmids
cryptic plasmids
all of these

⇒ Cos site of the cosmids
consists of 12 bases
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both (a) and (b)
contains cleavage site

⇒ M 13 is an example of
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single stranded DNA vector
both (a) and (b)
plasmid

⇒ A plasmid can be considered as a suitable cloning vector if
it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties
All of the above

⇒ Cryptic plasmids
do not exhibit any phenotypic trait
exhibit many phenotypic traits
exhibit one phenotypic traits
exhibit antibiotic resistance

⇒ λ ZAP vector is an example of
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phagemid
cosmid
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⇒ Stuffer is
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the left arm of the vector DNA
central fragment of the vector DNA
none of the above

⇒ PBR 322 has/have which of the following selection marker(s)?
Amp^r
Tet^r
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⇒ Charon vectors are different from EMBL vectors because
they have more extensive range of restriction targets with in their polylinkers.
physical separation of lambda arm from central fragment is required
both (a) and (b)
physical separation of lambda arm from central fragment is not required

⇒ Single stranded vectors are useful
for sequencing of cloned DNA
for oligonucleotide directed mutagenesis
for probe preparation
all of the above

⇒ Cosmids lack
genes coding for viral proteins
origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA

⇒ Inserted DNA in λ gt 11 can be expressed as
β-galactosidase fused protein
free protein in the cytoplasm
free protein that is secreted out
all of the above

⇒ Phagemid vectors are
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combination of phages and cosmid
phages carrying properties of plasmids
all of the above

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phages that lack cos site
plasmids that have no selection marker
cryptic plasmids

⇒ P1 cloning vector is the example of
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⇒ Phagemid consist of
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plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying origin of replication of plasmid only

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18 kb
50 kb
27 kb

⇒ Size of the DNA that can be packaged into a λ phage is
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