Question is ⇒ 5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is, Options are ⇒ (A) His 3, (B) Lys 2, (C) Ura 3, (D) Trp 1, (E) , Leave your comments or Download question paper.

Question is ⇒ 5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is, Options are ⇒ (A) His 3, (B) Lys 2, (C) Ura 3, (D) Trp 1, (E) , Leave your comments or Download question paper. Previous question Next question


Q1.	5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is
A.	His 3 [Wrong Answer]
B.	Lys 2 [Wrong Answer]
C.	Ura 3 [Correct Answer]
D.	Trp 1 [Wrong Answer]
View Answer 1 -1 Explanation:- Answer : C Discuss it below : !! OOPS Login [Click here] is required to post your answer/result Help other students, write article, leave your comments

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⇒ 5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is
His 3
Lys 2
Ura 3
Trp 1

⇒ Which of the following(s) is/are the most commonly used markers in yeast?
His 3
Ura 3
Trp 1
All of these

⇒ Recombinant SV40 viruses introduce the foreign DNA into cells
with the DNA mediated transfection
without the DNA mediated transfection
with the RNA mediated transfection
without the RNA mediated transfection

⇒ The size of the genome of SV 40 is
7.2 kb
69 kb
5.2 kb
110 kb

⇒ Moloney-Mu-leukemia virus is an example of
Vaccinia virus
Baculo virus
Retro virus
none of these

⇒ Which of the following viruses are used as vectors for transferring genes to animal cells?
SV 40 virus
Bovine papilloma virus (BVP)
Retroviruses
All of these

⇒ Cos cell line is
cohesive end site of phage λ
derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ
derivative of non-permissive CV-1 monkey cell line

⇒ Cloning genes in the yeast offer which of following advantage(s)?
Over production of proteins of commercial value
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA
All of the above

⇒ Maximum amount of foreign DNA introduced into SV 40 viruses is
10 kb
5 kb
2.5 kb
1 kb

⇒ Maximum insert size in case of adenovirus vectors is
6-8 kb
4-6 kb
10-12 kb
20 kb

⇒ Lytic infection of SV 40 does not occur in
permissive cells
non permissive cells
both (a) and (b)
none of these

⇒ SV 40 vectors, in which late region replacements are done, require
COS cell line
SV 40 helper virus
pBR 322 vector
none of these

⇒ Autonomously replicating sequence is not present in
YIp
YRp
YCp
YAC

⇒ Which of the following can be used for reducing the size of gene inserts containing introns?
Vaccinia virus
Baculo virus
Retro virus
none of these

⇒ Bovine papilloma virus has a circular double stranded DNA genome of about
2.5 kb
5.2 kb
7.9 kb
9.5 kb

⇒ Retroviruses have advantage for being used as vector for animal cells because
they cover a wide host range
infection does not lead to cell death, infected cells produce virus over an indefinite period
viral gene expression is driven by strong promoters
all of the above

⇒ Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries autonomously replicating sequence
Carries centromere and telomere sequences

⇒ In case of Baculoviruses, the foreign gene
is inserted downstream of polyhedrin promoter
is inserted downstream of viral origin of replication
is inserted between the polyhedrin promoter and downstream sequences
is inserted randomly in viral genome

⇒ Retroviruses consist of
Ds DNA genome
Ss DNA genome
RNA genome
cDNA genome

⇒ Which of the following promoters is repressed in presence of glucose?
Galactokinase (GAL 1)
Acid phosphatase (PHOS)
Both (a) and (b)
Phosphoglycerate kinase (PGK)

⇒ Which of the following histone is not present in SV40 virus?
H1
H2A
H3
H4

⇒ Which of the following are produced in high copy number?
YEp
YRp
Both (a) and (b)
YCp

⇒ Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
Vaccinia virus
Baculo virus
Papilloma virus
Retrovirus

⇒ Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries autonomously replicating sequence
Carries centromere and telomere sequences

⇒ SV 40 vectors, in which early region replacements are carried out, require
COS cell line
SV 40 helper virus
pBR 322 vector
none of these

⇒ Yeast Replicating plasmids (YRp) carry
centromere
telomere
autonomously replicating sequence
both (a) and (c)

⇒ YAC vectors can be used to obtain artificial chromosomes because it carries
centromere
telomere
autonomously replicating sequence
all of these

⇒ Which one of the following is the most stable vector?
YEp
YRp
YAC
YIp

⇒ Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
episomally only
integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number

⇒ Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
transcriptional regulators and are essential for viral replication
not necessary for viral replication
coding for virus coat protein
important for packaging of viral DNA into viral head