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Home⇒ Medical Science⇒ Biotechnology⇒ Animal cell culture and regulation
1-Dont try to cram or spend more time here just read it fast and cover syllabus then practice MCQ's cheptor of same topic to check your progress. . |
2-Wrong options are also given but dont concentrate there, Right answer is in bold format. |
Q21 ⇒ At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via oxidative pathway [other wrong options] [Discuss in forum] anaerobic pathway both (a) and (b) none of these |
Q22 ⇒ For culturing, plasma from the adult chicken is preferred to mammalian plasma because it forms a clear and solid coagulum even after dilution [other wrong options] [Discuss in forum] it is too opaque it doesn't produce solid clots it forms a semi solid coagulum |
Q23 ⇒ What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? Lactate helps in the growth while ammonium inhibits the growth [other wrong options] [Discuss in forum] They act as growth promoters They act as growth inhibitors Have no effect on cells |
Q24 ⇒ During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? The cells do not have enough oxygen [other wrong options] [Discuss in forum] Ethyl alcohol is being produced in excess The cells have too much oxygen Glycolysis is being inhibited |
Q25 ⇒ Range of optimum glucose concentration present in the culture media is 5.5 - 55 mmol/litre [other wrong options] [Discuss in forum] 55 - 75 mmol/litre 75-105 mmol/litre 105-150 mmol/litre |
Q26 ⇒ The major problem associated with the isolation of free cells and cell aggregates from organs is that of releasing the cells from their supporting matrix [other wrong options] [Discuss in forum] inhibiting the cells from their supporting matrix disintegrating the cells from their supporting matrix none of the above |
Q27 ⇒ According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of 12 amino acids [other wrong options] [Discuss in forum] 6 amino acids 8 amino acids 10 amino acids |
Q28 ⇒ Which of the followings are the metabolic products of glucose and glutamine? Lactate and ammonium [other wrong options] [Discuss in forum] CO2 and NH3 CO2 and lactate Lactate only |
Q29 ⇒ How does CO2 help in the cell metabolism during cell culture? It participates in the de novo synthesis of purines and pyrimidines [other wrong options] [Discuss in forum] Helps in the cells respiration For monitoring pH of the culture All of the above |
Q30 ⇒ Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called established cell lines [other wrong options] [Discuss in forum] primary cell lines secondary cell lines propagated cell lines |
Q31 ⇒ Accumulation of lactate leads to reduction in the pH of culture hence loss of cell viability [other wrong options] [Discuss in forum] increase in pH no change in pH no loss of cell viability |
Q32 ⇒ What is the concentration of CO2 required for culturing animal cells? 1-10% [other wrong options] [Discuss in forum] 2-5% 10-15% 15-20% |
Q33 ⇒ Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping? all of these [other wrong options] [Discuss in forum] Down's syndrome Turner's syndrome Klinefelter's syndrome |
Q34 ⇒ Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 0.05-5 pmol of O2/cell/h [other wrong options] [Discuss in forum] 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h |
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